{"id":835,"date":"2017-04-17T16:43:43","date_gmt":"2017-04-17T09:43:43","guid":{"rendered":"http:\/\/en.prc.maiatech.com.vn\/?p=835"},"modified":"2017-07-22T20:55:44","modified_gmt":"2017-07-22T13:55:44","slug":"chromatin-organization-and-cytological-features-of-carnivorous-genlisea-species-with-large-genome-size-differences","status":"publish","type":"post","link":"https:\/\/en.prc.org.vn\/?p=835","title":{"rendered":"Chromatin organization and cytological features of carnivorous Genlisea species with large genome size differences"},"content":{"rendered":"<p style=\"text-align: justify;\">Trung D. Tran<sup>1 <\/sup>, <sup>\u00a0<\/sup>Hieu X. Cao<sup>1<\/sup>, Gabriele Jovtchev<sup>1\u2020<\/sup>, Petr Nov\u00e1k<sup>2<\/sup>, Giang T. H. Vu<sup>1<\/sup>, Ji\u0159\u00ed Macas<sup>2<\/sup>, Ingo Schubert<sup>1,3<\/sup> and Joerg Fuchs<sup>1*<\/sup><\/p>\n<p style=\"text-align: justify;\"><sup>1<\/sup>Department of Breeding Research, Leibniz Institute of Plant Genetics and Crop Plant Research (IPK), Gatersleben, Germany<\/p>\n<p style=\"text-align: justify;\"><sup>2<\/sup>Institute of Plant Molecular Biology, Biology Centre of the Czech Academy of Sciences, \u010cesk\u00e9 Bud\u011bjovice, Czech Republic<\/p>\n<p style=\"text-align: justify;\"><sup>3<\/sup>Central European Institute of Technology and Faculty of Science, Masaryk University, Brno, Czech Republic<\/p>\n<p style=\"text-align: justify;\">The monophyletic carnivorous genus <em>Genlisea<\/em> (Lentibulariaceae) is characterized by a bi-directional genome size evolution resulting in a 25-fold difference in nuclear DNA content. This is one of the largest ranges found within a genus so far and makes <em>Genlisea<\/em> an interesting subject to study mechanisms of genome and karyotype evolution. <em>Genlisea nigrocaulis<\/em>, with 86 Mbp one of the smallest plant genomes, and the 18-fold larger genome of <em>G. hispidula<\/em> (1,550 Mbp) possess identical chromosome numbers (2<em>n<\/em> = 40) but differ considerably in chromatin organization, nuclear and cell size. Interphase nuclei of <em>G. nigrocaulis<\/em> and of related species with small genomes, <em>G. aurea<\/em> (133 Mbp, 2<em>n<\/em> \u2248 104) and <em>G. pygmaea<\/em> (179 Mbp, 2<em>n<\/em> = 80), are hallmarked by intensely DAPI-stained chromocenters, carrying typical heterochromatin-associated methylation marks (5-methylcytosine, H3K9me2), while in <em>G. hispidula<\/em> and surprisingly also in the small genome of <em>G. margaretae<\/em> (184 Mbp, 2<em>n<\/em> = 38) the heterochromatin marks are more evenly distributed. Probes of tandem repetitive sequences together with rDNA allow the unequivocal discrimination of 13 out of 20 chromosome pairs of <em>G. hispidula<\/em>. One of the repetitive sequences labeled half of the chromosome set almost homogenously supporting an allopolyploid status of <em>G. hispidula<\/em> and its close relative <em>G. subglabra<\/em> (1,622 Mbp, 2<em>n<\/em> = 40). In <em>G. nigrocaulis<\/em> 11 chromosome pairs could be individualized using a combination of rDNA and unique genomic probes. The presented data provide a basis for future studies of karyotype evolution within the genus <em>Genlisea<\/em>.<\/p>\n<p style=\"text-align: justify;\"><strong>Full text:<\/strong> <a href=\"https:\/\/prc.org.vn\/UserFiles\/File\/Bai%20bao%20khoa%20hoc\/Chromatin%20organization%20and%20cytological%20feature%20of%20carnivorous%20Genlisea%20species%20with%20large%20genome%20size%20differences.pdf\"><em>Chromatin organization and cytological features of carnivorous Genlisea species with large genome size differences<\/em><\/a><\/p>\n","protected":false},"excerpt":{"rendered":"<p>Trung D. Tran1 , \u00a0Hieu X. Cao1, Gabriele Jovtchev1\u2020, Petr Nov\u00e1k2, Giang T. H. Vu1, Ji\u0159\u00ed Macas2, Ingo Schubert1,3 and<\/p>\n","protected":false},"author":3,"featured_media":989,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":[],"categories":[39],"tags":[],"_links":{"self":[{"href":"https:\/\/en.prc.org.vn\/index.php?rest_route=\/wp\/v2\/posts\/835"}],"collection":[{"href":"https:\/\/en.prc.org.vn\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/en.prc.org.vn\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/en.prc.org.vn\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/en.prc.org.vn\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=835"}],"version-history":[{"count":2,"href":"https:\/\/en.prc.org.vn\/index.php?rest_route=\/wp\/v2\/posts\/835\/revisions"}],"predecessor-version":[{"id":990,"href":"https:\/\/en.prc.org.vn\/index.php?rest_route=\/wp\/v2\/posts\/835\/revisions\/990"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/en.prc.org.vn\/index.php?rest_route=\/wp\/v2\/media\/989"}],"wp:attachment":[{"href":"https:\/\/en.prc.org.vn\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=835"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/en.prc.org.vn\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=835"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/en.prc.org.vn\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=835"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}